mOTUs

3.0.3
A QIIME2 wrapper for mOTU-tool - taxonomical profiling of metagenomic samples.

install guide:

  1. Install and activate the latest QIIME2 environment.
conda activate qiime2-2022.8
  1. Run the following commands
git clone https://github.com/motu-tool/q2-mOTUs
cd q2-mOTUs
make install

mOTUs is a command line tool designed for taxonomic profiling of shotgun metagenomic samples. mOTUs allows you to profile known and currently unknown species using a set of 10 universal single copy marker genes. The input is metagenomic data generated from DNA that was extracted directly from an environmental sample (represented as one or more fastq files) and the output is a taxonomic profile identifying which species are present and quantifying their abundance. Now it is available within QIIME2 ecosystem!

For details, see the paper:
"Ruscheweyh, HJ., Milanese, A., Paoli, L. et al. Cultivation-independent genomes greatly expand taxonomic-profiling capabilities of mOTUs across various environments. Microbiome 10, 212 (2022). https://doi.org/10.1186/s40168-022-01410-z"

Usage example might be found at GitHub repository https://github.com/motu-tool/q2-mOTUs.

In case of questions, please post them on QIIME2 Forum.